Genome-editing of multiple copy genes in polypoloid Chrysanthemums
Advances in genome editing technology offer new hope for modifying traits in polyploid plants, especially those which lack whole genome information, such as Chrysanthemum morifolium (chrysanthemum) - a commercially important hexaploid plant that is propagated asexually worldwide. Kishi-Kaboshi et al. performed genome editing of transgenic chrysanthemums containing multiple copies of a fluorescent protein gene. They found that one genome-editing event was able to introduce mutations in several copies of the transgene, with mutations accumulating in vegetatively-propagated shoots and regenerated calli. Through this approach, multi-allelic genes, and thus polyploid plant traits, can be modified successfully.
The cover image shows fluorescence by transgenic chrysanthemum flowers expressing the yellowish-green fluorescent protein gene from Chiridius poppei (CpYGFP) (top), and three young shoots (bottom) are CpYGFP-chrysanthemum (left), partially mutagenized genome-edited CpYGFP-chrysanthemum (center), and wild type chrysanthemum (right). Images supplied by M. Kishi-Kaboshi and K. Sasaki (National Agriculture and Food Research Organization, Japan).