Nicotiana benthamiana is a member of the Solanaceae family and is widely used as an experimental model system in plant science, especially in the fields of plant pathology and for understanding interactions between organisms. However, N. benthamiana has a complex allopolyploid genome, the details of which have long been elusive. Kurotani et al. (on pp. 248-257) have now performed de novo whole-genome assembly in N. benthamiana using Hi-Fi technology with next-generation sequencing, resulting in improved genome assembly and annotation of N. benthamiana, which will undoubtedly render it more widely accessible for research.