cendo様

生理活性物質の抽出に関するご質問（登録番号1040）にお答えします。回答は東京大学・生物生産工学研究センターの山根久和教授を通して、帝京大学バイオサイエンス科の横田孝雄教授にしていただきました。また、山根教授からは、参考になる文献等をご教示いただきましたので、あわせて送ります。

　横田教授からのアドバイス：
「活性炭の小さなカラムに通すと色（クロロフィル）が抜けるかもしれません。色がでるようなら、抽出液に水を適量加えれば良いのではないでしょうか。。メタノール抽出物なら効率が良いと思われます。しかし、吸着したもののなかに活性物質がある場合にはだめですね。」

山根教授から：
下記の論文では、植物のメタノール抽出物を購入してスクリーニング源として用いているようです。色素を含んだ状態かもしれませんが、扱いやすいようになっているのかもしれません。

Biochem Biophys Res Commun. 2006 Aug 24; [Epub ahead of print]
RelatedArticles, Links
Selective LXRalpha inhibitory effects observed in plant extracts of MEH184(Parthenocissua tricuspidata) and MEH185 (Euscaphis japonica).
Kim KH, Choi SH, Lee TS, Oh WK, Kim DS, Kim JB.Department of Biological Sciences, Research Center for Functional　Cellulomics, Seoul National University, Seoul 151-742, Republic of Korea

MEH preparation. Three hundred plant extracts including MEH184 (P.tricuspidata) and MEH185 (E. japonica) were purchased from Plant Extract Bank, Plant Diversity Research Center, KRIBB (Daejeon, Korea). All botanicalsamples were identified and authenticated at Plant Extract Bank where allvoucher specimens have been deposited.

また、下記の論文のように関連研究を京都大学の大東先生のグループが行っているので、論文別刷を請求するか、京都大学グループに相談すしては如何でしょうか。

Antioxid Redox Signal. 2005 Nov-Dec;7(11-12):1621-9. （このジャーナルは東大では購入されていないようです。）

Suppressive effects of mioga ginger and ginger constituents on reactive　oxygen and nitrogen species generation, and the expression of inducible pro-inflammatory genes in macrophages.

Kim HW, Murakami A, Abe M, Ozawa Y, Morimitsu Y, Williams MV, Ohigashi H.　Division of Food Science and Biotechnology, Graduate School of Agriculture,　Kyoto University, Kyoto 606-8501, Japan.

We previously conducted screening tests of the chloroform extracts from a total of 89 species of Japanese plant food items for their suppressive effects on superoxide (O(2) ()) generation through both NADPH oxidase and xanthine oxidase, and reported that mioga ginger (Zingiber mioga Roscoe) indicated the strongest suppressive activities.
In this study, the suppressive effects of mioga ginger constituents, aframodial, and galanal B, together with [6]-gingerol and galanolactone occurring in ginger, on free radical generation and inducible proinflammatory gene expressions were investigated. Of these constituents, aframodial (20 microM) exhibited marked suppressive effects on 12-O-tetradecanoylphorbol-13-acetate-induced O(2) () generation in HL-60 cells and lipopolysaccharide (LPS)/interferon-gamma-induced nitric oxide (NO) generation in RAW264.7 cells (inhibition rates [IRs]=84.6% and 95.9%, respectively).
Aframodial also strongly suppressed the stimulated HL-60 cell-induced mutagenicity in AS52 cells (IR=95.9%). The LPS-induced expression of inducible proinflammatory genes such as inducible NO synthase, interleukin (IL)-1beta, IL-6, and granulocyte-macrophage colony-stimulating factor was significantly abolished (IRs=99.1%, 74.6%, 74.0%, and 64.4%, respectively) by aframodial. In addition, degradation of the inhibitor of nuclear factor kappaB was suppressed by this compound (IR=100%), suggesting that the suppression of nuclear factor kappaB activation, at least in part, is involved. Taken together, these results suggest that aframodial has potent antioxidative and anti-inflammatory potentials, and may be a promising candidate in prevention and/or therapy for chronic inflammationassociated carcinogenesis. Antioxid. Redox Signal. 7, 1621-1629.

山根 久和（東京大学・生物生産工学研究センター・環境保全工学部門）