The 63rd Annual Meeting of the Japanese Society of Plant Physiologists
March 22-24, 2022 Tsukuba International Congress Center
Outline
Symposia
◆S01 Improvement of genomics and technologies upgrades the value of bioresources
Date: 9:30-12:22, March 22 (Tuesday), 2022
Venue: Room Y
Organizers: Yutaka Saito (National Institute of Genetics), Masatomo Kobayashi (RIKEN BioResource Research Center)
Bioresources are an essential foundation for the life science research. Bioresources are used as model experimental systems and play an important role in standardizing experimental results. Another major feature of bioresources is that they shed light on the biological diversity.
The National BioResource Project (NBRP) is a government supported program for collecting, preserving, and providing bioresources that the government recognizes as important to strategically develop. NBRP plant resources include Arabidopsis/ cultured plant cells/ genes, rice, wheat, barley, tomato, Lotus and Glycine, morning glory, Chrysanthemum, and algae.
In recent years, with the advent of genomic information and new technologies, research utilizing various bioresources. In this symposium, we would like to consider the new utilization of NBRP's plant resources from the perspective of genomic information and new technology, and discuss the future of plant science research that transcends the boundaries of plant species.
Diverse Resources of Plant Cell Cultures and Quality Control for Their Advanced Utilization
Non-model algal resources provide insights into the unexpected features in eukaryotes
Development of a nested association mapping population of hexaploidy wheat: from genetic resources to genetic resources
Progress of chromosome scale genome assembly methods in barley
Utilization of wild rice genetic resources enabled by genome information and editing
Genomic basis for environmental adaptation of Lotus japonicus revealed by applying NBRP resources
Molecular Genetics in Chrysanthemum: A Model Strain and its Whole Genome Sequence
The Japanese morning glory: Our country’s unique bioresource shines brightly with genome information
NBRP Tomato Historia, Current and Future
◆S02 Plant resilience mechanism for irregular environmental fluctuations over time
Date: 9:30-12:30, March 22 (Tuesday), 2022
Venue: Room Z
Organizer: Motoyuki Ashikari (Nagoya University), Tomonao Matsushita (Kyoto University)
Plants are exposed to constantly fluctuating environments. Plants developed multiple stage gates that allow them to sense temporal and spatial environmental information so they can respond better and survive. What kind of stage gates did plants develop? How do plants respond under fluctuating environments? This symposium focuses on the molecular mechanisms underlying plant resilience to temporal fluctuating environments in the context of stage gates. (This symposium is sponsored by Grants-in-Aid for Scientific Research on innovative areas: Plant resilience under fluctuating environment.)
Regulatory mechanisms of plant growth phase transition in response to nitrogen nutrient deficiency
Stage-gates for response to submergence in roots
Stagegate of rice stem elongation under submergence
Plant resilience mechanism for heat stress
Regulation of stomatal movement and flowering under fluctuating environments
Molecular mechanism of wound-induced responses as a strategy of plant resilience
Robust regulation of a flowering repressor FLC under fluctuating environments
◆S03 The forefront of plant RNA molecular biology: Sequence, structure and function
Date: 13:45-16:40, March 22 (Tuesday), 2022
Venue: Room Y
Organizer: Yui Yamashita (Grad. Sch. Agr., Hokkaido Univ.), Masayuki Tsuzuki (Grad. Sch. Arts Sci., Univ. Tokyo)
In plants, RNA molecules have crucial roles in various processes, including growth, morphogenesis, development, and environmental response. Although a couple of decades have passed since the discovery of non-coding RNAs, the number of reports on novel molecular species, regulatory sequences and modifications is still growing. In addition to classical methods in molecular biology, recent advances in structural biology and bioinformatics have provided us with new insights into structure and function of RNA molecules. In this symposium, leading researchers in the field of plant RNA biology in Japan will introduce their latest research, and discuss our “view on plant” through RNA molecules with emphasis on their function, sequence, and structure.
How genes are transcribed and processed: the importance of UsnRNP function in plants
The mechanisms of secondary siRNA biogenesis in plants
The function and mechanism of non-coding RNA transcription in plants
Structures insight into the C-to-U RNA editing enzyme in plant organelles suggests a unique regulation principal
What are characteristics of plant NMD targets?
Coordinated regulation of translational and transcriptional expression of transporter genes in response to boron concentration
Mechanistic specificity of ribosome stalling involved in ER-stress response in plants
Detailed analysis of mRNA sequence revealed relationship between mRNA sequence variants and translational control
◆S04 Sensors and actuators in biology and architecture
Date: 13:45-16:45, March 22 (Tuesday), 2022
Venue: Room Z
Organizer: Masatsugu Toyota (Dept. Biochem. & Mol. Biol., Saitama Univ.), Haruko Ueda (Dept. Biol., Konan Univ.)
Both organisms and architectures maintain their structures and functions by detecting, processing and integrating external information. Plants and animals sense environmental stimuli such as gravity and light and change their growth, morphology and movement accordingly. Architectures keep their shapes against mechanical stresses such as earthquakes and storms. Although the size (μm to m) and the amplitude of the force (pN to kN) are different among plants, animals and architectures, their common feature is that they could detect the external stimuli with “sensors” and convert the information into motions with “actuators”. In this symposium, we will discuss the similarities and differences in the sensors and the actuators of organisms and architectures and find the fundamental principles.
Application Of Sensor And Actuator Technologies To Spatial Structures
Bio-inspired architecture
Dynamic microtubule reorganization as a sensor and actuator of growth fluctuation and stabilization
Photosensor-induced changes in leaf anatomy
Sensors and actuators regulating the fast movement of the Venus flytrap
How a growing brain is constructed: sensing and actuation by densely packed cells
◆S05 Toward understanding the unique features of plant stem cells
Date: 9:00-11:40, March 23 (Wednesday), 2022
Venue: Room Y
Organizer: Masaaki Umeda (NAIST), Hitoshi Sakakibara (Nagoya Univ.)
In animals, pluripotent stem cells disappear soon after early embryogenesis, while plant stem cells maintain pluripotency in tissues and function as a source of various organs. This feature enables continuous growth of plants. Another feature of plants is to have a survival strategy through somatic cell reprogramming. We launched a project supported by a Grant-in-Aid for Scientific Research on Innovative Areas in 2017, and have studied generation, proliferation and maintenance of plant stem cells, and reprogramming of somatic cells. In this symposium, the core group leaders will present the progress in the past five years, providing an opportunity for stepping forward to a new era in stem cell research. (This symposium is supported by a Grant-in-Aid for Scientific Research on Innovative Areas, entitled ‘Principles of pluripotent stem cells underlying plant vitality’.)
Genome Maintenance Strategies in Plant Stem Cells
Analyses of stem cell genome diversity in long-lived plants
Regulation of nodule initiation in legumes
Evolutionary conserved mechanisms of stem cell proliferation in land plants
Control of vegetative reproduction by KL signaling in Marchantia polymorpha
Role of cytokinin biosynthesis and translocation in the maintenance of shoot apical stem cells
Regulation of stem cell production by a cytochrome P450-derived signal in plants
Analysis on the establishment of apical-basal axis and stem cells in rice embryo
Deceleration of cell cycle underpins a switch from proliferative to terminal division in plant stomatal lineage
Competitive action among BES/BZR transcription factors enables the robust control of vascular stem cells
◆S06 Chemical signals that control parasitism, symbiosis, defense, and infection in plants
Date: 13:15-16:15, March 23, 2022
Venue: Room Y
Organizers: Masami Yokota Hirai (RIKEN CSRS, Grad. Sch. Bioagric. Sci., Nagoya Univ.), Masaharu Mizutani (Kobe University), Akifumi Sugiyama (RISH, Kyoto Univ.)
There are various interactions (parasitism, symbiosis, defense, infection, etc.) between plants and other organisms. In order to interact, it is necessary to recognize each other, but since both are spatially separated, it is necessary to sense the signals emitted from the individual to be recognized to the outside. These signals are often metabolites secreted by plants: for example, flavonoids and strigolactone are known to act as chemical signals in nodule formation and AM symbiosis, respectively. In many biological interactions, however, signals remain to be clarified. Since the initial response caused by a chemical signal is unique, the search for a new chemical signal requires a screening system using the initial response as an assay system and subsequent substance purification, and an approach different from conventional metabolic physiology research is expected. This symposium will focus on chemical signals related to the dynamic relationship in parasitism, symbiosis, defense, and infection between plants and other organisms and provide the latest topics on substance identification and physiological functions. In addition, future research developments will be discussed.
Mycorrhizal cheating mediated by signaling molecules
Regulation of arbuscular mycorrhizal fungi by plant-derived compounds
Parasitic strategies of Ralstonia solanecearum on fungi as well as plants
Identification of plant attractant of parasitic nematode, M. incognita
Plant defense system coordinated by herbivore’s oral secretion components
Counter-adaptation and physiological mechanisms of insects to gardenia defense substances
The mystery of Insect Gall formation is being elucidated
The influence toward undergraound interactions of plant communication on above ground by VOCs
◆S07 A multifaceted approach to uncovering the mechanism and dynamics of the plant-microbe holobiont
Date: 13:15-16:15, March 23, 2022
Venue: Room Z
Organizers: Shunsuke Miyashima (NAIST), Kei Hiruma (The Tokyo University), Akira Mine (Kyoto University)
Plants and microbes together form a collective assemblage called holobiont, which dramatically improves their environmental adaptability. In order to understand this plant-microbe holobiont, it is essential to decipher host-microbe interactions within the holobiont in a multidimensional manner and integrate the knowledge gained. In this symposium, we will present the achievements of researchers who are unraveling the plant-microbe holobiont from their unique research approaches, including in vivo imaging, modeling, and functional genomics. We would like to take the first step toward establishing a research community that unravels and manipulate plant-microbe holobiont.
Fluorescence lifetime imaging reveals spatiotemporal activation of immune response in Arabidopsis root
Minerals and photosynthates dynamics in root using radioisotope imaging
Development of quantification techniques to unravel the plant physiological response
Detecting beneficial organisms for rice by reconstructing ecological interaction networks under field conditions
Diagnosis and surveillance of wheat stem rust using field transcriptomic data
Plant-microbiota “remote” interactions via secreted compounds manipulating host root growthdefense coordination
◆The 18th Database Workshop
Date: 9:00-12:00, March 24 (Thursday), 2022
Venue: Room Z
Organizer: Kentaro Yano (Sch. of Agri., Meiji Univ.)
In the 18th Database workshop, we will focus on web databases, AI text-mining methods, machine/deep learning methods for large-scale omics data in plant science. At first, web databases for plant science, including the database RAP-DB providing the reference genome sequence and gene annotation of rice, will be introduced. To provide the latest knowledge about rice genes, the RAP-DB has been updating structural and functional information of genes based on literature information for over ten years. The current status of databases for the plant omics data will be discussed. Then, the large-scale integrated analysis with NGS sequencing data and online data will be shown. It allows us to efficiently and effectively detect genes and factors involved in a biological process of interest. Here, a wealth of highly reliable annotations about gene functions facilitates the identification of genes and proteins. Although reliable information can be collected from the published literature, manual curation is time consuming. In this workshop, the powerful approach with AI text-mining methods and knowledge-bases will be introduced. Finally, a Convolutional Neural Network (CNN) approaches will be discussed. Although relevance of the diagnosis by CNN (to say, “why did you say that” in CNN) had been in a black box in conventional technologies, recent methods for network backpropagation, so called “explainable AIs” allow for feature visualization of the diagnosis by CNN. User-friendly applications of deep learning image/DNA sequences diagnosis will be introduced, too.
Co-sponsored by Plant Informatics Society
Literature-based curation of gene annotation, genomic variations, and transcriptomics information in RAP-DB
Gene screening for plant spermatozoid formation using NGS data
Databases, ‘AI text mining’ and deep learning methods in plant science
Tips for “collaboration with AI”, to “wet” plant researchers